Coding

Part:BBa_K2560269:Design

Designed by: Memduha Muratoglu   Group: iGEM18_Marburg   (2018-10-02)


tfoX (V. natriegens)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the coding region from tfox of V. natriegens without the start codon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011). It was built with the Marburg Toolbox, a golden gate based toolbox for modular cloning. According to the Marburg Toolbox, the part is designed as a 4-part (CDS).

Iif there is no need for tagging, use this part.

GGTCTCGAATG-coding_region-GCTTTGAGACC


Source

genomic DNA of ATCC 14048

References