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Part:BBa_K2560133:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-10-08)


Phytobrick version of KanRes in BBa_K2560002

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 146
    Illegal XhoI site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by polymerase chain reaction (PCR) on the template BBa_K2560057 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGTGCTAAACGGGCAAGGTGTCACC

Reverse Oligo: TTCGTCTCCCTCATGTTAAGATGCGTGATCTGATCCTTC

Source

As Source DNA we used BBa_K2560057 from our Toolbox.