Phytobrick version of BBa_B1006
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The sequence was obtained from part BBa_B1006. The parts sequence was not changed apart from adding the terminator overhangs that are required for subsequent cloning.
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGGCTTAAAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTTCGCT
Reverse Oligo: CTCAAGCGAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTTTAAGC