Phytobrick version of Tetracyclin Resistance
This plasmid can be used to create Tetracyclin resistance parts for the "Marburg Collection".
An antibiotic resistance is the ability of a microorganism to resist the effect of an antibiotic (WHO). Specially microorganisms can acquire this property when they are exposed to low, non-lethal antibiotic concentrations e.g. when stopping an antibiotic treatment too early. In molecular and synthetic biology resistances are used as markers to select transformed organisms. Fully functional cassettes are coded on commercial available plasmids setting a selection pressure on the transformants when platting them on agar plates containing the corresponding antibiotics in an appropriate concentration.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids.
36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk.
The Marburg Collection contains 123 parts in total, including:
inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.
Parts of the Marburg Toolbox
Tags and Entry Vectors