Phytobrick version of dCas9
This is the Phytobrick version of the coding sequence dCas9 and was build as a part of the Marburg Collection. Instructions of how to use the Marburg Collection are provided at the bottom of the page.
Open reading frames (ORFs) are sequences that can theoretically be translated into functional proteins. They are predicted by a start codon (ATG) at their beginning and a stop codon (TAA, TAG, TGA) at the end separated by multiple codons. When an ORF is confirmed to code for a functional protein it is classified as a coding sequence (CDS). CDSs code only proteins. To translate them they need a promoter upstream the start codon.A terminator downstream of the sequence end the translation. In prokaryotes an RBS sequence is needed between the promoter and the start codon. In eukaryotes CDSs are flanked by untranslated regions (UTRs). In addition eukaryotic CDSs contain introns which are removed after transcription by splicing to form the mature mRNA which is translated into the functional protein outside the nucleus.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1107
- 21Illegal BamHI site found at 3386
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1
Illegal BsaI.rc site found at 4118
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids.
36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk.
The Marburg Collection contains 123 parts in total, including:
inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.
Parts of the Marburg Toolbox
Tags and Entry Vectors