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Part:BBa_K2550202

Designed by: Gaurav Byagathvalli   Group: iGEM18_Lambert_GA   (2018-10-15)


Stem-Loop Domain for Toehold Switch Design

Toehold switches are RNA-based riboregulators functioning as switches that can activate/inhibit reporter expression using a synthetic construct. In the presence of a reverse complementary RNA trigger sequence, reporter expression can be activated through the binding of the trigger sequence to the toehold switch, resulting in the uncoupling of the secondary structure, allowing the RBS to be freed from the "closed" position, allowing for translation of the reporter protein. Toehold switches are advantageous as arbitrary sequences can be utilized to regulate expression of a reporter, serving as unique tools for incorporation as biosensors.

The functionality of toehold switches are dependent on the secondary structure through a stem-loop domain. This arbitrary sequence is responsible for maintaining the "closed" position of toehold switches until uncoupling occurs following binding of the trigger sequence. This structure is critical for the optimization of any toehold switch design.

This part encodes an arbitrary sequence producing a stem-loop structure, which when coupled with the target sequence of choice and complementary parts, can form a complete toehold switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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