- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BamHI site found at 299
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 774
The constitutive promoter is BBa_J23100; the RBS sequence is BBa_B0034; rhlR (LVA tagged) is BBa_C0071; the random spacer is from the plasmid pET28a; the terminator is BBa_B0015.
The rhlR sequence comes from P. aeruginosa PA3477 with LVA tag. rhlR protein acts as transcriptional regulator and binds with N-butyryl-HSL (C4-HSL).