Plasmid_Backbone

Part:BBa_K2546009

Designed by: Tsu-Wang Sun   Group: iGEM18_NCHU_Taichung   (2018-10-12)


kan bbrf2

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 4193
    Illegal suffix found at 915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4193
    Illegal NheI site found at 3
    Illegal NheI site found at 26
    Illegal SpeI site found at 916
    Illegal PstI site found at 930
    Illegal NotI site found at 923
    Illegal NotI site found at 4199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4193
    Illegal BamHI site found at 3044
    Illegal XhoI site found at 3177
    Illegal XhoI site found at 4069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4193
    Illegal suffix found at 916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4193
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4208
    Illegal SpeI site found at 916
    Illegal PstI site found at 930
    Illegal NgoMIV site found at 1308
    Illegal AgeI site found at 1148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 701


[edit]
Categories
Parameters
None