Plasmid_Backbone

Part:BBa_K2546008

Designed by: Tsu-Wang Sun   Group: iGEM18_NCHU_Taichung   (2018-10-12)


pME60C2 shuttle vector

This part is a shuttle vector with green fluorescence protein (GFP). It is composed by the Ori of pBBR1 and BBa_J364007 in pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5466
    Illegal suffix found at 915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5466
    Illegal NheI site found at 3
    Illegal NheI site found at 26
    Illegal SpeI site found at 916
    Illegal PstI site found at 930
    Illegal NotI site found at 923
    Illegal NotI site found at 3580
    Illegal NotI site found at 5472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5466
    Illegal BamHI site found at 4317
    Illegal XhoI site found at 4450
    Illegal XhoI site found at 5342
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5466
    Illegal suffix found at 916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5466
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5481
    Illegal SpeI site found at 916
    Illegal PstI site found at 930
    Illegal NgoMIV site found at 1308
    Illegal AgeI site found at 1148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 701
    Illegal BsaI.rc site found at 3902


[edit]
Categories
Parameters
None