Cold-inducible RNA-based thermosensor-6
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The pseudoknot structure is important to the function of cold-inducible RNA-based thermosensors. We designed the thermosensor by changing base pairing, base pair position and GC content in the pseudoknot region.
The natural cspA gene sequence is from Escherichia coli. The sequence we used is the 5'UTR of cspA. And we changed base pairing, base pair position and GC content of this sequence. We synthesized this sequence from a synthesis company.
Giuliodori A M, Di P F, Marzi S, et al. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.[J]. Molecular Cell, 2010, 37(1):21-33.
Breaker R R. RNA Switches Out in the Cold[J]. Molecular Cell, 2010, 37(1):1-2.
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.