Heat-inducible RNA-based thermosensor-36
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The secondary structure is important to the function of an RNA-based thermosensor. We designed the thermosensor according to the stem length, loop size and mismatches or bulges in the stem.
This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.
Neupert J, Karcher D, Bock R. Design of simple synthetic RNA thermometers for temperature-controlled gene expression, in Escherichia coli.[J]. Nature Protocols, 2008, 4(9):1262-73.
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.
Neupert J, Bock R. Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria. Nature Protocols, 2009, 4(9):1262-73.
Sen S, Apurva D, Satija R, et al. Design of a Toolbox of RNA Thermometers[J]. Acs Synthetic Biology, 2017, 6(8).