MelA expression construct
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1390
- 1000Illegal BsaI site found at 1242
Illegal BsaI.rc site found at 547
the melA cds was assembled with an T7 promoter that can be induced with IPTG in E. coli BL21(DE3) cells, and included with an E. coli RBS and terminator.
DNA synthesized by IDT based on the Rhizobium etli gene sequence.