Plasmid_Backbone

Part:BBa_K2448038:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


pSB1C3 LacIq

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3218
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3218
    Illegal XhoI site found at 2202
    Illegal XhoI site found at 3094
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3218
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3218
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3233
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3218
    Illegal XbaI site found at 3233
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


Design Notes

Source

LacIq sequence was amplified by PCR from the pQLinkH vector [1] and inserted using the Golden Gate Assembly technique into the pSB1C3 bearing the BBa_J04450 reporter.

References

[1] Calos MP. DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation. Nature (1978) 274, 762-765.

[2] Scheich C, Kümmel D, Soumailakakis D, Heinemann U, Büssow K. Vectors for co-expression of an unrestricted number of proteins. Nucleic Acids Research (2007) 35, e43.