D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens
Usage and Biology
D-Psicose 3-epimerase (EC: 22.214.171.124) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.
The A. tumefaciens Dpe is a 31.56 kDa protein of 289 amino acids.
This enzyme is highly specific for D-Psicose, but it is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose and D-sorbose . For optimal activity, it requires cofactors such as Mn2+ or Co2+, although it has a low basal activity without ions. The native Dpe shows a tetrameric arrangement of 132 kDa, and each subunit has 289 amino acids with a molecular weight of 33 kDa. The topology of each subunit is a TIM-barrel fold with a cluster of eight β-strands surrounded by twelve α-helices .
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 208
Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 619
Illegal AgeI site found at 646
- 1000Illegal SapI.rc site found at 174