Reporter

Part:BBa_K2448004:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This codon optimized mCherry for Escherichia coli K12 is derived from (BBa_J06504). The optimization has been performed with help of JCAT.

Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024)

Source

DNA synthesis

References