Reporter

Part:BBa_K2448003:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


LacZ-alpha (pUC19 like)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pUC19 DNA sequence encoding LacZ-alpha was modified with the help of JCAT in order to remove the numerous cloning site present especially in the MCS of pUC19.

Source

DNA synthesis

References

[1] Juers DH, Matthews BW, Huber RE. LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci. (2012) 21, 1792-1807.