Coding

Part:BBa_K2443066

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)


EF-G

Elongation factor G is a prokaryotic translocase and GTPase responsible for catalyzing and coordinating the movement of tRNA and mRNA through the ribosome. It has been Codon optimized for use in Escherichia coli and contains an N-terminal Histidine tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 580
    Illegal AgeI site found at 1219
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1869
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 1990
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None