Composite
ACCswitch

Part:BBa_K2434008

Designed by: Santiago Ochoa   Group: iGEM17_TecMonterrey_GDA   (2017-10-17)


Regulated ACC synthesis

The ACC gene encodes for Acetyl-CoA Carboxylase, which has been shown to increase the synthesis of fatty acids. But an overexpression of ACC leads to a lack of carbon necessary for the Krebs cycle. In order to avoid this problem ACC is controlled by the pBad promoter, allowing for a normal metabolism in the absence of arabinose. The iLOV protein can be used as a reporter for the expression of ACC.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1210
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1758
    Illegal SapI.rc site found at 1955


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Categories
Parameters
None