Designed by: Freiburg Bioware09 Group: iGEM09_Freiburg_bioware (2009-10-14)
Middle Linker ( Gly-Gly-Ser-Gly)x2
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.
The sequence has no cleavage site for proteases.
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file
Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.