geneIII unter control of a T7 promoter
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 29
Illegal BglII site found at 1386
Illegal BamHI site found at 721
Illegal XhoI site found at 1372
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution  or related systems .
The part is fully compatible with RFC10, as well as with Golden Gate assembly
Bacteriophage T7, Bacteriophage M13, with modifications.
 Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.
Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.