Regulatory

Part:BBa_K2398555:Design

Designed by: Lukas Platz   Group: iGEM17_Heidelberg   (2017-10-26)


Organosilicon binding Riboswitch

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is only 54 base pair long nucleotide sequence. Therefore it is recommended to order two complementary oligos with overhangs corresponding to restriction overhangs used in golden gate assembly. This is also the reason for not submitting this part. To functionalize this part you have to anneal both oligos in a Touchdown-PCR approach.

Source

This part has no source whatsoever. It is derived from in silico models for the binding of organosilicons.

References