Coding

Part:BBa_K2389050

Designed by: Rochelin Dalangin   Group: iGEM17_UAlberta   (2017-10-26)


mCherry1.5

mCherry1.5 is a recently published red-shifted variant of mCherry with a longer Stokes shift and less cytotoxicity to cells. We were hoping to use it as part of our enrichment procedure, where a decrease in fluorescence over successive rounds of enrichment would indicate successful enrichment for colonies producing gas vesicles.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None