RBS

Part:BBa_K2355219:Design

Designed by: Philip SÝrensen   Group: iGEM17_DTU-Denmark   (2017-10-27)


Strong promoter with RFC[25] scars and a startcodon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We has to make this part, with added RFC[25] scars, as the composite part system on the registry doesn't allow us to add our own sequences to a composite part, and because the registry system automatically assumes that the RFC[10] standard was used for 3A assembly, even though the RFC[10] will create uneven scars, thereby introducing a frame-shift in any fusion protein assembled this way.


Source

Part BBa_B0030 was used as the RBS in this part

References