Coding

Part:BBa_K2351014

Designed by: Giel Scheepers   Group: iGEM17_Utrecht   (2017-10-23)


dAsCpf1 - PhytoBrick

Please note: PhytoBrick in Universal Acceptor Plasmid (BBa_P10500)
Prefix fusionsite: 1234 = AATG // Suffix fusionsite: 5678 = GCTT
See BBa_K2351001 for illegal BioBrick version and BBa_K235101 for codon-corrected BioBrick version.

CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015). Containing D908A mutation to render it 'dead'. It still has its DNA and gRNA binding properties however, the endonuclease activity is gone. Codon optimized for mammalian cell types.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BglII site found at 1919
    Illegal BglII site found at 2228
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
    Illegal NgoMIV site found at 3463
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None