note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351001 for illegal BioBrick version and BBa_K2351011 for PhytoBrick version.
CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015). Containing D908A mutation to render it 'dead'. It still has its DNA and gRNA binding properties however, the endonuclease activity is not active.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 923
Illegal XhoI site found at 1111
Illegal XhoI site found at 2525
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 2209
- 1000Illegal BsaI.rc site found at 2094
Illegal SapI site found at 408