DNA

Part:BBa_K2351011

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-23)


dAsCpf1

note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351001 for illegal BioBrick version and BBa_K2351011 for PhytoBrick version.

CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015). Containing D908A mutation to render it 'dead'. It still has its DNA and gRNA binding properties however, the endonuclease activity is not active.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 923
    Illegal XhoI site found at 1111
    Illegal XhoI site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2094
    Illegal SapI site found at 408


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Categories
Parameters
None