DNA

Part:BBa_K2351010

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-23)


secreted Cpf1

note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351006 for illegal BioBrick version and BBa_K2351012 for PhytoBrick version.


This sequence codes for the CRISPR-associated protein Cpf1 but is being secreted out of the cell due to a signal sequence fused to the N-terminus of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 977
    Illegal XhoI site found at 1165
    Illegal XhoI site found at 2579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2148
    Illegal SapI site found at 462


[edit]
Categories
Parameters
None