note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351006 for illegal BioBrick version and BBa_K2351012 for PhytoBrick version.
This sequence codes for the CRISPR-associated protein Cpf1 but is being secreted out of the cell due to a signal sequence fused to the N-terminus of the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 977
Illegal XhoI site found at 1165
Illegal XhoI site found at 2579
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 2263
- 1000Illegal BsaI.rc site found at 2148
Illegal SapI site found at 462