DNA

Part:BBa_K2351009

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-23)


secreted Cas9

note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351005 for illegal BioBrick version and BBa_K2351013 for PhytoBrick version.

This sequence codes for the CRISPR-associated protein Cas9 but is being secreted out of the cell due to a signal sequence fused to the N-terminus of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 570
    Illegal BglII site found at 915
    Illegal BglII site found at 1242
    Illegal BamHI site found at 3432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2240
    Illegal BsaI site found at 3794
    Illegal BsaI.rc site found at 902
    Illegal SapI site found at 202
    Illegal SapI site found at 1707


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Categories
Parameters
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