note: This part is codon corrected to remove illegal restriction sites, not experimentally validated.
For experimental validation, see BBa_K2351005 for illegal BioBrick version and BBa_K2351013 for PhytoBrick version.
This sequence codes for the CRISPR-associated protein Cas9 but is being secreted out of the cell due to a signal sequence fused to the N-terminus of the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1153
- 21Illegal BglII site found at 570
Illegal BglII site found at 915
Illegal BglII site found at 1242
Illegal BamHI site found at 3432
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 2240
Illegal BsaI site found at 3794
Illegal BsaI.rc site found at 902
Illegal SapI site found at 202
Illegal SapI site found at 1707