Protein_Domain

Part:BBa_K2351008

Designed by: Ouafa Dahri, Giel Scheepers   Group: iGEM17_Utrecht   (2017-10-23)


nCpf1

This BioBrick codes for the CRISPR-associated protein Cpf1 but does not have the first methionine and stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 920
    Illegal XhoI site found at 1108
    Illegal XhoI site found at 2522
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2206
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2091
    Illegal SapI site found at 405


[edit]
Categories
Parameters
None