Protein_Domain

Part:BBa_K2351007

Designed by: Ouafa Dahri, Giel Scheepers   Group: iGEM17_Utrecht   (2017-10-23)


nCas9

This BioBrick codes for the CRISPR-associated protein Cas9 but does not have the first methioinine and stopcodon. Instead, it can be used as a middle domain of a fusion protein by for example adding an N-terminal signal sequence [BBa_K2351000] and a his-tag [BBa_K2351004 ]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 858
    Illegal BglII site found at 1185
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 3737
    Illegal BsaI.rc site found at 845
    Illegal SapI site found at 145
    Illegal SapI site found at 1650


[edit]
Categories
Parameters
None