DNA

Part:BBa_K2351006

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-23)


Secreted Cpf1

note: This part is contains illegal restriction sites.
See BBa_K2351012 for PhytoBrick version and BBa_K2351010 for codon corrected (but unvalidated) BioBrick version.

This sequence codes for the CRISPR-associated protein Cpf1 but is being secreted out of the cell due to a signal sequence fused to the N-terminus of the protein. Signal sequence had to be designed to get the protein out of the cell. Also, the sequence has been codon optimized for mammalian cells such that glycosylation has been minimized to preserve protein function. As a result, this biobrick contains illegal restriction-sites. A new version that is compatible has been constructed and has this number: BBa_K2351010. In addition, this brick was uploaded as a phytobrick and the sequence component was validated as BBa_K2351012

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 1312
    Illegal PstI site found at 3721
    Illegal PstI site found at 3919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 1312
    Illegal PstI site found at 3721
    Illegal PstI site found at 3919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 434
    Illegal BglII site found at 1976
    Illegal BglII site found at 2285
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 1312
    Illegal PstI site found at 3721
    Illegal PstI site found at 3919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 1312
    Illegal PstI site found at 3721
    Illegal PstI site found at 3919
    Illegal NgoMIV site found at 3520
  • 1000
    COMPATIBLE WITH RFC[1000]


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