DNA

Part:BBa_K2351003

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-20)


nCpf1

This CRISPR-associated protein from Acidaminococcus sp. BV3L6 was used as a component of a secreted fusion protein. Protein does not contain first Methionine and stop codon. However, this BioBrick contains illegal restriction sites. For a similar BioBrick without illegal restriction sites, go to part: BBa_K2351008.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BglII site found at 1919
    Illegal BglII site found at 2228
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 193
    Illegal PstI site found at 1255
    Illegal PstI site found at 3664
    Illegal PstI site found at 3862
    Illegal NgoMIV site found at 3463
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None