DNA

Part:BBa_K2351002:Design

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-13)


Cas9 without first Methionine and without stopcodon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 2179
    Illegal NgoMIV site found at 2252
    Illegal NgoMIV site found at 2737
    Illegal NgoMIV site found at 3646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Methionine and stopcodon were taken out


Source

Streptococcus pyogenes


References