DNA

Part:BBa_K2351002

Designed by: Ouafa Dahri   Group: iGEM17_Utrecht   (2017-10-13)


Cas9 without first Methionine and without stopcodon

This BioBrick can be used as a component in secreted fusion proteins. However, this BioBrick contains an illegal restriction site. For a corrected sequence use BioBrick with number BBa_K2351007.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 2179
    Illegal NgoMIV site found at 2252
    Illegal NgoMIV site found at 2737
    Illegal NgoMIV site found at 3646
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None