
DNA
Part:BBa_K2351002
Designed by: Ouafa Dahri Group: iGEM17_Utrecht (2017-10-13)
Cas9 without first Methionine and without stopcodon
This BioBrick can be used as a component in secreted fusion proteins. However, this BioBrick contains an illegal restriction site. For a corrected sequence use BioBrick with number BBa_K2351007.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 787
Illegal PstI site found at 2209
Illegal PstI site found at 2413
Illegal PstI site found at 2443
Illegal PstI site found at 3655 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 787
Illegal PstI site found at 2209
Illegal PstI site found at 2413
Illegal PstI site found at 2443
Illegal PstI site found at 3655 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 787
Illegal PstI site found at 2209
Illegal PstI site found at 2413
Illegal PstI site found at 2443
Illegal PstI site found at 3655 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 787
Illegal PstI site found at 2209
Illegal PstI site found at 2413
Illegal PstI site found at 2443
Illegal PstI site found at 3655
Illegal NgoMIV site found at 1075
Illegal NgoMIV site found at 2179
Illegal NgoMIV site found at 2252
Illegal NgoMIV site found at 2737
Illegal NgoMIV site found at 3646 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |