Coding

Part:BBa_K2351001

Designed by: Giel Scheepers   Group: iGEM17_Utrecht   (2017-09-29)


dAsCpf1

note: This part is contains illegal restriction sites.
See BBa_K2351014 for PhytoBrick version and BBa_K2351011 for codon corrected (but unvalidated) BioBrick version.

CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015). Containing D908A mutation to render it 'dead'. It still has its DNA and gRNA binding properties however, the endonuclease activity is gone. Codon optimized for mammalian cell types. As a result, this BioBrick contains illegal restriction sites. For corrected codon version, see BBa_K2351011. In addition, this brick was uploaded as a phytobrick and the sequence component was validated as BBa_K2351014. . Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 380
    Illegal BglII site found at 1922
    Illegal BglII site found at 2231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 1258
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
    Illegal NgoMIV site found at 3466
  • 1000
    COMPATIBLE WITH RFC[1000]


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