mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 (see the full reference on the "Design" page). mScarlet-I is distinguished by a single amino acid substitution T74I from the original mScarlet protein. It possesses a 569nm absorbance and 594nm emission, and is notable for its high quantum yield (0.54) and fluorescence lifetime (3.1ns), as well as its rapid maturation time of ~36 minutes.
An improved mScarlet-I is available in part BBa_K2680251. This part is compatible with 3G assembly and can be used with William and Mary 2018's protocols , to produce large numbers of multi-transcriptional unit circuits and circuit variants.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC