mScarlet-I no stop codon
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Stop codons have been removed for the purpose of combination with protein degradation tags. The sequence for this part has been codon-optimized for E. coli.
mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT.
We use the website www.jcat.de to optimize the codon sequence for E. coli.
 Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074