Coding

Part:BBa_K2329003

Designed by: Karl Alex Hedin   Group: iGEM17_Chalmers-Gothenburg   (2017-09-19)


The GPCR Ri7

This part was never submitted due to time constraints.

Ri7, is an olfactory receptor from Rattus norvegicus and recognizes n-octanal. Previous studies have shown that Ri7 can successfully be incorporated into S. cerevisiae [1]. Check out Team: Chalmers-Gothenburg Achievements: Project results for a fully study of the GPCR.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 775
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 795



References

[1] Minic J, Persuy M-A, Godel E, Aioun J, Connerton I, Salesse R, and Pajot-Augy E. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS Journal, 272(2):524-537, 2005.

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