gRNA for Cas9 targeting STE2 in S. cerevisiae
The sequence for the promoter SNR52 and gRNA that creates a deletion in STE2. STE2 encodes the alpha-factor pheromone receptor . The gRNA successfully created a deletion on STE2.
BBa_K2329001 is a central part of our project, as replacing the native GPCR STE2 is vital to ensure that the only GPCR present on the yeast cell is the heterologous GPCR introduced by us.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 134
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
1. STE2 | SGD [Internet]. Yeastgenome.org. 2017 [cited 7 October 2017]. Available from: https://www.yeastgenome.org/locus/S000001868