Part:BBa_K2328067

LPP-OMPA + linker.b

Usage

The Lpp-OmpA consists of the first nine N-terminal amino acids of major E. coli lipoprotein (Lpp) bined with a transmembrane domain (46-159 aa) from outer membrane protein A (OmpA). With the help of Lpp-OmpA, we can direct different polypeptides to the cell surface of E. coli, such as β-lactamase, cellulases, etal. Now we hope to display our PETase on the surface of E. coli by using it.

Biology

Surface expression of recombinant proteins was first described more than 30 years ago.The Lpp-OmpA consists of the first nine N-terminal amino acids of major E. coli lipoprotein (Lpp) bined with a transmembrane domain (46-159 aa) from outer membrane protein A (OmpA). We got its sequence from the genome of Escherichia coli str. K-12 substr.

Reference

[1] Ali Karami, Ali Mohamad Latifi*, and Samaneh Khodi，etal. 2014. Comparison of the Organophosphorus Hydrolase Surface Display Using InaVN and Lpp-OmpA Systems in Escherichia coli. J. Microbiol. Biotechnol. (2014),24(3), 379–385 [2] Joseph A. Francisco*, etal. 1992. Transport and anchoring of β-lactamase to the external surface of Escherichia coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]