Composite

Part:BBa_K2323001:Design

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)


Leptotrichia wadei Cas13a with a His-Tag and a SUMO-tag for protein purification.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1736
    Illegal BglII site found at 3416
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2815


Design Notes

This composite part includes a SUMO-Tag to improve the purification efficiency by improving solubility and allows the cleaving of the His-Tag after purification.


Source

Leptotrichia wadei

References

J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)