Leptotrichia wadei Cas13a with a His-Tag and a SUMO-tag for protein purification.
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1736
Illegal BglII site found at 3416
Illegal BamHI site found at 112
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 2815
This composite part includes a SUMO-Tag to improve the purification efficiency by improving solubility and allows the cleaving of the His-Tag after purification.
J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)