Part:BBa_K2314515
GH1-1 ( β-glucosidase) coding sequence
This part is a gene which encoding β-glucosidase. The β-glucosidase capable of hydrolyzing cellobiose into glucose. β-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and oligosaccharides, with release of glucose. β-glucosidases catalyze the hydrolysis of soluble cellodextrins to glucose and are a critical component of cellulase systems. In our project,we expressed the GH1-1 and CDT-1 in EBY100, the combinations of CDT-1 with GH1-1 constitute fully functional cellodextrin transport systems.We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1115
Illegal BamHI site found at 726
Illegal XhoI site found at 322
Illegal XhoI site found at 985 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 236
Illegal BsaI site found at 376
Illegal BsaI.rc site found at 1030
Illegal SapI site found at 993
Illegal SapI.rc site found at 1377
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