DNA

Part:BBa_K2308005

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-11)


coding sequence of sYFP2 knock in for fusion expression with puhA and detection

This part is used to knock in the coding sequence of sYFP2 with puhA for fusion expression in Rhodobacter Sphaeroides, using the method of redET. The part is composed of 5 basic parts(BBa_K2308008,BBa_K2308009,BBa_K2308003,BBa_K2308011,BBa_K2308007) If the gene is knocked in by homologous recombination, the strain will be selected by Amp resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
    Illegal BamHI site found at 2197
    Illegal XhoI site found at 96
    Illegal XhoI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1965


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Categories
Parameters
None