Coding

Part:BBa_K2294444:Design

Designed by: Mathias Gotsmy   Group: iGEM17_BOKU-Vienna   (2017-09-25)


green fluorescent protein derived from jellyfish Aequeora victoria GFP RFC1000 compatible

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence complies to every RFC standard (http://parts.igem.org/partsdb/scars.cgi). The specific fusion sites are created as suggested (http://2014.igem.org/Team:BostonU/MoClo), AATG overhang at the 5' end, GCTT overhang at the 3' end.

Source

This GFP CDS is derived from Cormack 1996 (http://www.sciencedirect.com/science/article/pii/0378111995006850).

References

For the original sequence see http://parts.igem.org/Part:BBa_E0040.