Coding

Part:BBa_K2294444

Designed by: Mathias Gotsmy   Group: iGEM17_BOKU-Vienna   (2017-09-25)


green fluorescent protein derived from jellyfish Aequeora victoria GFP RFC1000 compatible

This is an improved RFC[1000] compatible version of the BBa_E0040 GFP part (http://parts.igem.org/Part:BBa_E0040).

The changes are:

1) A silent mutation to remove a BsaI recognition site.

2) RFC[1000] specific prefix and suffix with CDS fusion sites (AATG in the 5' end; GCTT in the 3' end).



Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None