AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD
This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team
This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 2615
- 21Illegal BglII site found at 576
Illegal BamHI site found at 2555
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 408
Illegal AgeI site found at 1093
- 1000Illegal BsaI site found at 1096
|n/a||AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD|