This is a branch of the AND GATE built by Peking University 2009 iGEM Team. The addition of salicylate can induce the transcription of T7ptag. However, if the SupD tRNA is absent, T7ptag might not be translated into functional T7 polymerase normally because of some amber stop codon mutations in it. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC