The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated.
The complementary information and the structure of the tRNA is predicted by DNAman as shown in fig1.
This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 33
- 1000Illegal BsaI site found at 36
Reference Anderson JC, Voigt CA, and Arkin AP. Environmental signal integration by a modular AND gate. Molecular Systems Biology. 2007, 3:133