Minimal SpyCatcher codon optimized for Bacillus subtilis with His Tag
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
|Prefix with||EcoRI, NotI, XbaI, NgoMIV and SD||GAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGGCCGGC|
|Suffix with||AgeI, SpeI, NotI and PstI||ACCGGTTAATACTAGTAGCGGCCGCTGCAGA|
Sites of restriction enzymes generating compatible overhangs are indicated by sharing one color. (EcoRI and PstI are marked in blue, NotI in green, XbaI and SpeI in red and AgeI and NgoMIV in orange. Additionally, the Shine-Dalgarno sequence is marked in silver and the start and stop codon is underlined.)
The His Tag was added to the sequence via PCR with BioBrick BBa_K2273015 as a template using the primers listed below.
Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.