This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
In this case, the constitutively expressed LuxR transcriptional regulator part1: http://parts.igem.org/Part:BBa_K2267029 （we improved） is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
http://parts.igem.org/Part:BBa_K2267040 is Control，
we did point mutation on http://parts.igem.org/Part:BBa_R0062 and the exact spots are
listed below. We linked gfp reporter genes luxr-plux1-gfp: http://parts.igem.org/Part:BBa_K2267043
on them respectively to test the function of modified promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860
- 21Illegal BglII site found at 928
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2179