Composite

Part:BBa_K2258003

Designed by: Jyothish S   Group: iGEM17_IISER-Pune-India   (2017-10-27)


pCAT_AmilCP

It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)

Usage and Biology

This part is useful as constitutively expressed a reporter.

T--IISER-Pune-India--ChromophorePellet.jpg T--IISER-Pune-India--LiquidCulture.jpg

iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).


Photometric Characterization

T--IISER-Pune-India--AmilCPabsorbance.jpg

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.


T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part
//cds/reporter/chromoprotein
//chassis/prokaryote
Parameters
biologyconstitutive reporter
chassisE.coli
colorBlue
emission590nm